Anatoluin A and B isolated from medicinal Tricholoma anatolicum are new cytotoxic ergostanoids against the most common cancers

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Kaplaner E., Aydoğmuş-Öztürk F., Öztürk M., AKATA I., Duru M. E.

Natural Product Research, cilt.37, sa.22, ss.3787-3797, 2023 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 37 Sayı: 22
  • Basım Tarihi: 2023
  • Doi Numarası: 10.1080/14786419.2022.2153360
  • Dergi Adı: Natural Product Research
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aquatic Science & Fisheries Abstracts (ASFA), Biotechnology Research Abstracts, CAB Abstracts, Chemical Abstracts Core, CINAHL, EMBASE, Food Science & Technology Abstracts, MEDLINE, Veterinary Science Database
  • Sayfa Sayıları: ss.3787-3797
  • Anahtar Kelimeler: Tricholoma anatolicum, isolation and elucidation, cytotoxic activity, cancer cell lines, fibroblast cell lines, lanostane steroids (ergosterols), EDIBLE MUSHROOMS, CONSTITUENTS, TRITERPENOIDS, EXTRACTS
  • Ankara Üniversitesi Adresli: Evet

Özet

© 2022 Informa UK Limited, trading as Taylor & Francis Group.Tricholoma anatolicum is an edible mushroom from the matsutake group growing under Cedar trees. Bioactivity-guided fractionation of Tricholoma anatolicum afforded two new (1 and 2), three known ergosterols (3–6), and four known (6–9) compounds. Structures were identified as anatoluin A (1), anatoluin B (2), 5α,6α-epoxy-ergosta-7,22-dien,3β-ol (3), ergosterol-endoperoxide (4), ergosterol,3β-ol (5), 3,5-dihydroxyfuran-2(5H)-one (6), mannitol (7), turanose (8), fumaric acid (9) using spectroscopic techniques. The cytotoxic activity of extract and isolated compounds was performed using MTT assay against MCF7, HT29, H1299, and HeLa cancerous cell lines while toxicity against PDF and L929 fibroblast healthy cell lines. The lipid peroxidation inhibitory and ABTS•+ scavenging activities were used to determine antioxidant activity. The polar extracts exhibited significant cytotoxic activity. The more perfect is that the extracts and isolated compounds (1–5) were inactive against PDF and L929 healthy cell lines. Compounds 1–3 and 4 exhibited noticeable cytotoxic activity, while 1–5 moderately inhibited lipid peroxidation.